Respuesta :
Answer:
The correct answers are: Dilution factor = 250 and concentration of DNA in undiluted sample = 2.5 μg/μl.
Explanation:
- Volume of DNA taken = 2 μl.
- Volume of added water = 498 μl.
- Total volume of the sample = 2 μl + 498 μl = 500 μl.
- Dilution factor = [tex]\frac{Final Volume}{Aliquot volume} = 500\div2 = 250.[/tex]
- A₂₆₀ of the given sample is = 0.2.
- We know that 50 μg/ml concentration of pure double stranded DNA gives an absorbance, A₂₆₀ = 1.
- Again, Concentration of DNA(µg/ml) = [tex]A_{260}\times \texrm{dilution factor}\times 50[/tex] µg/ml.
- = [tex]0.2\times\ 250\times 50 = 2500[/tex] μg/ml.
- In order to convert it into μg/μl, we have to divide the above value by 1000, hence we get, [tex]2500\div1000 =2.5[/tex] μg/μl.
